Journal: Materials Today Bio

Article Title: Engineering charge density in s-block potassium single-atom nanozyme for amplified ferroptosis in glioblastoma therapy

doi: 10.1016/j.mtbio.2025.101889

Figure Lengend Snippet: (a) The CLSM images of GL261 cells treated with Cy5.5-labeled K-SAN. (b) The CLSM images of GL261 cells colocalization. (c) The cell viability of non-cancerous cells after 24 h treatment under different K-SAN concentrations. (d) Tumor cell viability after 24 h of incubation with varying concentrations of K-SAN. (e) The fluorescence images of live/dead staining for GL261 cells incubated with varying formulations. (f) Flow cytometry measurements of GL261 cells after incubation with different formulations. ∗∗∗∗P < 0.0001.

Article Snippet: Laser irradiation further enhanced the green fluorescence intensity in the K-SAN group, while the red fluorescence intensity exhibited an inverse pattern.

Techniques: Labeling, Incubation, Fluorescence, Staining, Flow Cytometry

Journal: Materials Today Bio

Article Title: Engineering charge density in s-block potassium single-atom nanozyme for amplified ferroptosis in glioblastoma therapy

doi: 10.1016/j.mtbio.2025.101889

Figure Lengend Snippet: (a) DCF fluorescence images of GL261 cells following varying treatments. (b) The fluorescence images of •OH probe O26-stained GL261 cells following varying treatments. (C) The GSH levels in GL261 cells following varying treatments. (d)The CLSM images of MMP in GL261 cells following varying treatments. (e) Bio-TEM of GL261 cells treated with K-SAN. ∗∗∗∗P < 0.0001.

Article Snippet: Laser irradiation further enhanced the green fluorescence intensity in the K-SAN group, while the red fluorescence intensity exhibited an inverse pattern.

Techniques: Fluorescence, Staining

Journal: Materials Today Bio

Article Title: Engineering charge density in s-block potassium single-atom nanozyme for amplified ferroptosis in glioblastoma therapy

doi: 10.1016/j.mtbio.2025.101889

Figure Lengend Snippet: (a) The percentages of hemolysis in response to different concentrations of K-SAN (Inset: photograph of centrifuge tubes containing the supernatant from erythrocytes exposed to DI water or different concentrations of K-SAN in PBS). (b) The fluorescence images and (c) corresponding quantification of GL261 tumor-bearing mice at various time points following the injection of Cy5.5-labeled K-SAN. (d) The thermal images and (e) corresponding tumor temperature variations upon laser irradiation. (f) The body weight curves of mice following varying treatments. (g) The tumor growth curves of GL261 tumor-bearing mice following varying treatments. (h) The images and (i) tumor weights were collected from mice at the conclusion of the treatment. (j) The Kaplan-Meier survival profiles of mice following varying treatments. (k) H&E, (l) TUNEL staining of tumor slides were harvested from varying groups. (m) The immunohistochemical analysis of 4-HNE levels in tumors obtained from varying groups. (n) The immunofluorescence staining of GPX4 expressions and (o) ROS levels. ∗∗∗∗P < 0.0001.

Article Snippet: Laser irradiation further enhanced the green fluorescence intensity in the K-SAN group, while the red fluorescence intensity exhibited an inverse pattern.

Techniques: Fluorescence, Injection, Labeling, Irradiation, TUNEL Assay, Staining, Immunohistochemical staining, Immunofluorescence

Journal: JACC: Basic to Translational Science

Article Title: Large-Scale Functional Characterization of Low-Density Lipoprotein Receptor Gene Variants Improves Risk Assessment in Cardiovascular Disease

doi: 10.1016/j.jacbts.2024.10.006

Figure Lengend Snippet: HepG2 Cell Model for Characterization of LDLR Variants (A) Schematic presentation of the low-density lipoprotein receptor (LDLR) knockout cell line. (B) Representative image fields showing fluorescent low-density lipoprotein (DiI-LDL) uptake in HepG2 wild-type (WT) and LDLR knockout (KO) cells in lipid-poor (LP) conditions. (C) Quantification of mean DiI-LDL intensity and average number of DiI-LDL–filled organelles per cell in HepG2 WT and LDLR KO cells in 3 treatment conditions, lipid rich (LR), lipid poor (LP), and lipid poor plus mevastatin (LS), from over 10,000 cells from 3 independent experiments. (D) Illustration depicting the stable reintroduction of the LDLR wild-type green fluorescent protein (WT-GFP) gene into the genome of LDLR KO cells at the AAVS1 locus. (E) Representative image field showing stable expression of LDLR WT-GFP in LDLR KO cells. (F) Logarithmic density plot displays green fluorescent protein (GFP) signal intensities for KO-LDLR-WT-GFP–expressing and LDLR KO cell lines. The dashed line indicates the threshold value for discriminating GFP-positive from GFP-negative cells. (G) Box plot for GFP-positive and GFP-negative cells quantified from LDLR KO and LDLR WT-GFP–expressing cell lines, from over 15,000 cells from 5 independent experiments. (H) Representative image field demonstrating the restoration of DiI-LDL uptake in LDLR WT-GFP–expressing knockout cells with a zoom in of the indicated area for DiI-LDL and LDLR-GFP channels. Quantification of (I) mean DiI-LDL intensity, (J) average DiI-LDL–filled organelles per cell, and (K) mean LDLR WT-GFP intensity in HepG2 WT, LDLR KO, and KO-LDLR WT-GFP cells in LR, LP, and LS conditions. Over 15,000 cells from 3 independent experiments were quantified. Data were analyzed using the Mann-Whitney U test. P values were corrected for 7 and 11 tests for the analysis displayed in C and I, J, and K, respectively. Asterisks denote significance levels after Bonferroni correction for multiple comparisons: ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. A.U. = arbitrary units; cDNA = complementary DNA; LDL = low-density lipoprotein; pEF = Elongation Factor-1 alpha promoter.

Article Snippet: Mean green fluorescent protein (GFP) intensities for individual LDLR variants were distinct from nonexpressing LDLR KO cells but more variable as compared with LDLR WT-GFP ( ).

Techniques: Knock-Out, Expressing, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Ammonia-induced miRNA expression changes in cultured rat astrocytes

doi: 10.1038/srep18493

Figure Lengend Snippet: Cultured rat astrocytes were transfected with either miRNA inhibitors targeting rno-miR-221-3p, rno-221-5p, 222-3p or -326-3p or without inhibitor (control) and DNA content was quantified 48 h after seeding of the cells by fluorimetric detection of Hoechst34580 fluorescence as described in materials and methods. Fluorescence intensities measured in miRNA inhibitor-treated astrocytes are given relative to transfection control. (A) Effect of miRNA inhibition on astrocyte proliferation. (B) Effect of HO-1 inhibition by tin protoporphyin IX (SnPP, 10 μmol/l) on proliferation in miRNA inhibitor-treated astrocytes. *statistically significantly different compared to transfection controls. n.s.: not statistically significantly different as compared to SnPP-treated astrocytes. Data are from 3–4 independent experiments.

Article Snippet: Gene expression levels were quantified by measuring Bryt ® Green (Promega, Mannheim, Germany) fluorescence intensities using the 7500 real-time PCR system (Applied Biosystems®, Life Technologies, Darmstadt, Germany).

Techniques: Cell Culture, Transfection, Control, Fluorescence, Inhibition

Journal: Scientific Reports

Article Title: Ammonia-induced miRNA expression changes in cultured rat astrocytes

doi: 10.1038/srep18493

Figure Lengend Snippet: Cultured rat astrocytes were exposed to NH 4 Cl (5 mmol/l) or were left untreated (control) for 72 h in the presence or absence of taurine (5 mmol/l, 16 h pretreatment) or tin protoporphyrin IX (SnPP, 10 μmol/l, 30 min pretreatment). DNA content was quantified by fluorimetric detection of Hoechst34580 fluorescence as described in materials and methods and fluorescence intensities found under the different experimental treatments are given relative to the untreated control. *statistically significantly different compared to untreated controls. n.s.: not statistically significantly different. Data are from 3 independent experiments.

Article Snippet: Gene expression levels were quantified by measuring Bryt ® Green (Promega, Mannheim, Germany) fluorescence intensities using the 7500 real-time PCR system (Applied Biosystems®, Life Technologies, Darmstadt, Germany).

Techniques: Cell Culture, Control, Fluorescence